The acid-extractable, water-soluble, polysaccharide material of the brown alga Sargassum linifolium has been fractionated by using lead acetate and barium hydroxide, cetylpyridinium chloride, and copper acetate, and by chromatography on DEAE-cellulose and DEAE-Sephadex. A neutral, laminaran-like glucan and a sulphated heteropolysaccharide (sargassan), composed of D-glucuronic acid, D-mannose, D-galactose, D-xylose, L-fucose, and a protein moiety, were obtained. The latter polysaccharide could not be freed from protein. On partial hydrolysis, sargassan afforded three neutral oligosaccharides, together with a dialysable acidic fragment, a non-dialysable water-soluble portion, and the protein moiety. The backbone of sargassan seems to be composed of glucuronic acid, mannose, and galactose residues with partially sulphated side-chains composed of galactose, xylose, and fucose residues. Sargassan showed high anticoagulant activity.
acid-extractable、水溶性多糖物质的棕色海藻马尾藻类海草linifolium已经被使用醋酸铅和氢氧化钡,分馏氯化十六烷吡啶,醋酸铜,通过色谱DEAE-cellulose DEAE-Sephadex。一个中立,laminaran-like葡聚糖和硫酸化杂多糖(sargassan),由D-glucuronic酸,D-mannose,D-galactose,D-xylose,L-fucose,和一部分蛋白质,得到。后者无法摆脱蛋白多糖。部分水解,sargassan提供三个中性寡糖,连同dialysable酸性片段,non-dialysable水溶性部分,一半的蛋白质。的支柱sargassan似乎是由葡萄糖醛酸、甘露糖、半乳糖残基与部分硫酸化side-chains由半乳糖、木糖、岩藻糖残基。Sargassan显示高抗凝活性。