中文 » 英语<~ 复制Phytic acid enzymatic exist widely in plant, microbe, animals, broad prospect of application. In this paper 400123 for the primitive strains aspergillus, using the environment.molybdophosphoric blue colorimetric method for determination of live phytic acid enzymatic index, bran solid culture for matrix, in a single factor experiment, application based on the response surface method optimization design solid training process parameters, and the phytic acid enzymes in preliminary research enzymology character. Results show that bran matrix 1.24%, materials, water add ammonium sulfate than 1.5, corn starch 5.49%, pH 7.26 for optimal conditions, on the optimized conditions, 28 ℃ thermostatic cultivation 5d aspergillus solid-state fermentation phytic acid enzymes in 278.5400 U/g, da with theory predicts 266.9403 U/g match; This enzyme optimal reaction temperature of 40 ℃, pH 6.5; Fe2 +, metal ion Ca2 +, Mg2 +, add certain concentration making + for the enzymes are represented, including a activation function respectively the optimal additives mmol/L, 5 1.5 mmol/L, 1.5 mmol/L, 0.5, mmol/L than September calcium ion performance for the inhibition. The experimental result for the aspergillus phytic acid production development and application of enzymes to provide theoretical reference.
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